Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
AdipoGen PBS Endotoxin-free (sterile), 10 mL
Synonyms: Phosphate Buffer Saline Endotoxin-free (sterile). Endotoxin free buffer used for the dilution of TLRpure LPS and solubilisation of endotoxin-free reagents (e.g. ODNs).
Teknova 1X PBS with 0.05% Tween 20, 1% BSA, 1000mL, Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Sigma Aldrich Fine Chemicals Biosciences TrizmaR hydrochloride solu
A series of Pre-mixed SOLUTIONons of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy /- 0.1 pH units.
Kd Medical Inc PBS 20X Solution, PH 7.4, 1L
Phosphate Buffered Saline in a 20X concentrate for use as a wash buffer in immuno-labeling techniques. pH 7.4 One 1 Liter bottle.
Apexbio Technology LLC Protease Inhibitor Cocktail (EDTA-Free 100X in DMSO) 100x1ml
Crude cell extracts contain a variety of endogenous enzymes capable of degrading proteins such as phosphatases and proteases The optimal method to enhance the yield of intact proteins is to supplement with inhibitors targeting these known enzymes This Protease Inhibitor Cocktail is used in plant cell and tissue extracts to improve protein stability The cocktail inhibits proteases that degrade both non-phosphorylated and phosphorylated protein substrates The Protease Inhibitor Cocktail contains individual components including AEBSF 1 10-Phenanthroline Bestatin E-64 Leupeptin and Pepstatin A providing broad specificity against cysteine serine aspartic metalloproteases and aminopeptidases This protease inhibitor cocktail has been optimized and tested specifically for plant cell and tissue extracts Provided as a ready-to-use solution this protease inhibitor cocktail is supplied in DMSO as the solvent
![Alkali Scientific PBS [Phosphate Buffered Saline], 10X Powder Concentrate, White Granular Powder, 98.6g make, 1 L](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661308.jpg-250.jpg)
Alkali Scientific PBS [Phosphate Buffered Saline], 10X Powder Concentrate, White Granular Powder, 98.6g make, 1 L
PBS [Phosphate Buffered Saline] 10X Powder Concentrate is a white granular powder that, when dissolved in water, creates a 10X concentrated PBS solution. This 98.6g pack makes 1 liter of 10X PBS, providing a convenient and efficient way to prepare large volumes of phosphate-buffered saline. The powder dissolves quickly and is ideal for laboratories that need a concentrated PBS solution for dilutions or experiments that require higher concentration buffers. PBS is widely used in cell culture, molecular biology, and protein studies, and the 10X concentrate is ideal for reducing storage space and simplifying buffer preparation in the lab.
Sigma Aldrich Fine Chemicals Biosciences HEPES potassium salt | 82207-62-3 | MFCD00043438 | 25 g
HEPES potassium salt | Purity: ≥99.5% | Mol Wt: 276.39 | 82207-62-3 | MFCD00043438 | 25 g
Gold Biotechnology Inc HEPES Free Acid 2 x 1 kg
HEPES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al It is a zwitterionic piperazinic buffer that is useful for a pH range of 6 8 8 2 HEPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions
Boston Bioproducts Inc HEPPS Buffer (0.2 M, pH 8.0) - 250ml
HEPPS Buffer (0 2 M pH 8 0) - 250ml (For Research Use and Further Manufacturing Only)
![Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 500 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659833.jpg-250.jpg)
Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 500 G
HEPES, Free Acid is a commonly used biochemical reagent available in a 500g size for large-scale buffer preparation. This acid form of HEPES is highly effective in maintaining pH in the range of 6.8 to 8.2, making it ideal for biological applications where pH stability is crucial. It is commonly used in cell culture, protein analysis, and enzyme assays, ensuring that the pH remains constant over extended periods. HEPES is non-toxic to most cells, making it an excellent choice for maintaining physiological conditions during experiments. This bulk package is perfect for high-volume laboratories and applications that require frequent buffer preparation.
Bioworld PIPES BUFFER 1M PH 6.0 100ML
liquid, 1.0m, ph 6Synonym(s): 1,4-Piperazinediethanesulfonic acidMolecular Formula: C8H18N2O6S2CAS Number: 5625-37-6Molecular Weight: 302.37
Alkali Scientific Murashige & Skoog Medium with MES Buffer and Vitamins, 245 Grams of Powder, Makes 50 Liters of Solution
Murashige & Skoog (MS) Medium with MES Buffer and Vitamins is a high-quality powdered formulation used for plant tissue culture. This 245g powder makes 50 liters of a nutrient-rich solution, providing all essential macronutrients, micronutrients, and growth hormones. The addition of MES buffer ensures a stable pH environment for long-term culture, while the B5 vitamins promote cell growth and differentiation. This medium is ideal for in vitro propagation, genetic transformation, and plant regeneration studies. It is widely used in plant biotechnology labs for various applications including cloning, gene editing, and somatic embryogenesis.
Bioworld BES (2-(Bis(2-hydroxyethyl) amino) ethanesulfonic acid) Buffer, 0.5M, pH 6.5, 250 mL
Synonym: N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium salt, BES sodium saltBelongs to the class of Good buffers, as described in the research of Dr. Norman Good et al. (1966), optimized for molecular biology and biochemistry applications.The characteristics of a Good′s buffer include: pKa between 6.0 and 8.0, high solubility, non toxic, limited effect on biochemical reactions, very low absorbance between 240-700 nm, enzymatic and hydrolytic stability, minimal changes due to temperature and concentration, limited effects due to ionic or salt composition of the solution, limited interaction with mineral cations and limited permeability of biological membranes.Technical Information :pKa (25 °C) :7.1 (Free acid)Warning: For laboratory use only.Not fit for agricultural, household, clinical use or human consumption.Appearance: LiquidPH Value: 6.5
![Bioworld ACES (N-[carbamoylmethyl]-2-Aminoethane-sulfonic acid) Buffer, 0.5M, pH 7.0, 250 mL](https://assets.fishersci.com/TFS-Assets/CCG/product-images/50255317.jpg-250.jpg)
Bioworld ACES (N-[carbamoylmethyl]-2-Aminoethane-sulfonic acid) Buffer, 0.5M, pH 7.0, 250 mL
Synonym:N-(2-Acetamido)-2-aminoethanesulfonic acid; N-(Carbamoylmethyl)-2-aminoethanesulfonic acid; N-(Carbamoylmethyl)taurine ACES is one ofGood's buffersdeveloped in the 1960s to provide buffers withpHranging from 6.15-8.35 for use in various applications. Azwitterionicbuffer with apKaof 6.9, it is often used as abuffering agent inbiologicalandbiochemicalresearch. ACES had been used to develop buffers for bothagaroseandpolyacrylamide gel electrophoresis.A saturated solution contains 0.22 M at 0°C. ACES binds Cu2+, but not significantly Mg2+, Ca2+or Mn2+. Appearance: LiquidHS Code : 29241900Melting Point: >220 °C (dec.)(lit.)PH Value: 7.0