Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
National Diagnostics TRIS-GLYCINE ELECTROBLOTTING B
Tris-Glycine Electroblotting Buffer (10X), Stable, economical, ultra pure transfer buffer for Western Blotting procedures. 18 megOhm water. 0.2 micron filtration, 1 LT
Teknova 1M MES, pH 5.76. 1000mL, Sterile 2 pack.
MES solution is a zwitterionic buffer used to help maintain pH levels in biochemistry experiments. It has a buffering range of pH 5.8 - 6.5.
National Diagnostics SEQUAGEL BUFFER 200 ML
SequaGel Buffer, System consists of SequaGel Concentrate, SequaGel Diluent, and SequaGel Buffer for casting 19:1 denaturing DNA gels of a varying percentage, 200 ML
National Diagnostics ProtoGel Resolving Buffer (4X) 450ML
Traditional Laemmli Resolving Gel Buffer (4X). Formulated with 0.2 Micron Filtration.Ultra-Pure Reagents in 18 MegOhm Water.Crystal Clear, Reproducible Gels. Catalog number: EC-892. The use of National Diagnostics' ProtoGel Resolving Buffer will ensure the purity and performance of your Laemmli gels. When diluted as directed ProtoGel Resolving Buffer forms a gel of 0.375 M Tris-HCl and 0.1% SDS, pH 8.8. Storage: ProtoGel Resolving Buffer is stable for 24 months when stored tightly capped in a dark area at room temperature.
Sigma Aldrich Fine Chemicals Biosciences HEPES BioUltra, for molecular biology, 99.5 (T), 7365-45-9, MFCD00006158
HEPES BioUltra, for molecular biology, 99.5 (T)
![Research Products International Corp MES Monohydrate [2-(N-Morpholino)-ethanesulfonic acid], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M22040-100.0.JPG-250.jpg)
Research Products International Corp MES Monohydrate [2-(N-Morpholino)-ethanesulfonic acid], 100 Grams
Buffer for use in plant tissue culture.
- pKa (20°C): 6.15
- pH range: 5.5 - 6.7
Sigma Aldrich Fine Chemicals Biosciences Trizma base, 77-86-1, MFCD00004679, 500g
Linear Formula NH2C(CH2OH)3, ≥99.9% (titration), Molecular Weight 121.14, bp 219-220 °C/10 mmHg(lit.), mp 167-172 °C (lit.), Primary Standard and Buffer, crystalline, Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS 98.0 (titration), 6976-37-0, MFCD00002853
BIS-TRIS 98.0 (titration)
Boston Bioproducts Inc HEPES Buffer (0.5 M, pH 7.4) - 250ml
The HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)] Buffer is a versatile Good's zwitterionic buffering agent widely used in various applications in biochemistry and molecular biology. It maintains a stable pH in the range of 6.8 to 8.2, making it ideal for various applications at physiological pH, including cell culture, protein purification, isoelectric focusing, enzyme studies, bioprocessing, and live cell imaging.
Teknova 1M Tris-HCl, pH 6.5. 1000mL, Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Alkali Scientific CellPro™ MOPS, 10X, Brown Plastic Bottle, 1L, 12/cs
Alkali Scientific™ CellPro™ MOPS Buffer 10X is a versatile reagent commonly used in molecular biology and biochemistry applications. With its optimized formulation, this buffer provides stable pH conditions, crucial for nucleic acid electrophoresis, RNA isolation, and protein gel electrophoresis. Each batch undergoes stringent sterile filtration to ensure purity and consistency, making CellPro™ MOPS Buffer 10X a trusted choice for researchers seeking reliable results in their experiments.
Alkali Scientific CellPro™ 1M HEPES pH 7.5 (Adjusted with KOH) 1000mL
CellPro™ 1M HEPES pH 7.5 (Adjusted with KOH) 1000mL