Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Cell Signaling Technology Phosphate Buffered Saline (PBS-1X) pH7.2 (Sterile) 10 ml
Phosphate Buffered Saline (PBS-1X) pH7.2 (Sterile) 10 ml
Zymo Research Corporation ZymoPURE™ Binding Buffer (150 ml)
ZymoPURE™ Binding Buffer (150 ml). Assists in binding nucleic acid to ZymoPURE™ columns for purification.
Rockland Immunochemicals 10X TE pH 7.5 (100 mM Tris HCl 10 mM EDTA) DEPC TREATED - MB-006
TE buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA, cDNA or RNA. "TE" is derived from its components: Tris, a common pH buffer, and EDTA, a molecule that chelates cations like Mg2+. The purpose of TE buffer is to solubilize DNA or RNA, while protecting it from degradation.
HANNAS PHARMACEUTICAL SUPPLY CO INC NORMOSOL-R PH 6.6 12X1000ML
NC2125588 NORMOSOL-R PH 6.6 12X1000ML
Bioworld BES-Buffered Saline (2X), 250 mL
Product DescriptionBES (N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid) saline buffer has a concentration of 2X and a pH of 7.1 at 25°C. BES is a zwitterionic buffer used in biochemistry and molecular biology, and has been used to study versatile catalyst precursors for mild hydrocarboxylation of alkanes to carboxylic acids. BES was used to determine that saline buffers minimize the action of interfering factors in the bacterial endotoxins test. This saline buffer contains 50 mM BES, 1.5 mM Na2HPO4, and 280 mM NaCl.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
Hampton Research HR2-900-11/StockOptions Tris #11 pH 8.0, 185 ml
HR2-900-11/StockOptions Tris #11 pH 8.0, 185 ml. Please note items from this supplier are non-returnable.
Biotium Fixation Permeabilization Other Buffers DNA Gel Stains and Accessories DNA Gel Stains Chemical Reagents 50/PK
TAE (Tris-acetate-EDTA) buffer powder packet for preparing 1 L of 1X TAE buffer for nucleic acid gel electrophoresis Box includes 50 packets
Polysciences, Inc. Tris Buffered Saline; Composition: 1M Tris-HCl, 3M Sodium chloride; 8.0+/-0.2 pH; Storage: 25 deg.C
Tris Buffered Saline; Composition: 1M Tris-HCl, 3M Sodium chloride; 8.0+/-0.2 pH; Storage: 25 deg.C
Teknova 100mM Tris-HCl, pH 8.2. 1000mL, Sterile 6 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Teknova 50mM Tris HCl, pH 7.5, 1000mL, DNase, RNase, Endotoxin Tested. Sterile. 8 pack.
50mM Tris HCl, pH 7.5, 1000mL, DNase, RNase, Endotoxin Tested. Sterile. 8 pack.
Growcells PBS 20X 10L EA
Sterile 20X Phosphate-Buffered Saline solution concentrate prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: NaCl 160g, KCl 4g, Na2HPO4 28.8g, KH2PO4 4.8g